Easy-Spin (DNA free) Total RNA Extraction Kit

RNA isolation / purification Tissue - Mouse Cornea

Experiment

RNA isolation / purification Tissue - Mouse Cornea

Product

Easy-Spin (DNA free) Total RNA Extraction Kit from iNtRON Biotechnology

Manufacturer

iNtRON Biotechnology

Buy product Write review

Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- If lysate is allowed to overheating during homogenization, lysate can be placed on ice.	- For ethanol contamination, ensure that during the Washing buffer B, the easy-spinTM column is spun at maximum speed 1min to dry the easy-spinTM column

Protocol tips
- If lysate is allowed to overheating during homogenization, lysate can be placed on ice.
Downstream tips
- For ethanol contamination, ensure that during the Washing buffer B, the easy-spinTM column is spun at maximum speed 1min to dry the easy-spinTM column

Publication protocol

Total RNA was isolated from tissues using the Easy-Spin Total RNA extraction kit (GeneAll, Seoul, Korea). Following incubation with RNase-free DNase I (Promega, Madison, WI, USA), reverse transcription was performed using a random primer and MultiScribe™ MuLV reverse transcriptase (Applied Biosystems, Foster City, CA, USA) according to the manufacturer’s instructions

Full paper Login or join for free to view the full paper.

Reviews

Easy-Spin (DNA free) Total RNA Extraction Kit from iNtRON Biotechnology has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Comment View 2 comments

Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

Comment View 2 comments

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Mouse Cornea using Easy-Spin (DNA free) Total RNA Extraction Kit from iNtRON Biotechnology.

Paper title

Effects of nicotine on corneal wound healing following acute alkali burn

Full paper

Manufacturer protocol

Download the product protocol from iNtRON Biotechnology for Easy-Spin (DNA free) Total RNA Extraction Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Tissue - Mouse Cornea using Easy-Spin (DNA free) Total RNA Extraction Kit from iNtRON Biotechnology. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

Outsource experiment