Lipofectamine® 2000 Transfection Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Do not add antibiotics to the Transfection Medium

Protocol tips
Do not add antibiotics to the Transfection Medium

Publication protocol

Four synthetic siRNA sequences designed to inhibit expression of Myh10 (nonmuscle myosin IIB) were made to order by SABiosciences (Frederick, MD) (Table 1). SureSilencing siRNA Myh10 (SIM133594A, B, C, D) probes were each resuspended to 12.5 nmol in nuclease‐free DEPC‐treated water (Ambion; Austin, TX) before they were combined with Lipofectamine 2000. The four sequences of Myh10 (A–D) served as reagents for siRNA knockdown of nonmuscle myosin IIB. GAPDH (SIM202946A, Table 1) served as the siRNA control in Myh10 knockdown experiments to ensure that the process of siRNA infection did not cause non‐specific inhibition of Myh10 probes (A–D).

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Lipofectamine® 2000 Transfection Reagent below.

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