PureLink™ RNA Mini Kit

RNA isolation / purification Tissue - Mouse Colon

Experiment
RNA isolation / purification Tissue - Mouse Colon
Product
PureLink™ RNA Mini Kit from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- Depending on the material you want to isolate RNA from, please refer to the manual for additional reagents/lab equipment that are required
Downstream tips
- DNase is not supplied with this kit, a DNase kit is available which is compatible with this kit

Publication protocol

Ten milligrams of tissue mass was used to obtain high quality, DNA-free, RNA with Pure Link RNA Mini Kit (Ambion, Carlsbad, CA, U.S.A.) according to manufacturer’s instructions, to ensure high-quality DNAse solution was applied to each sample and traces of DNAse were eliminated by additional washing steps.

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Reviews

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Discussion

Discussion

1 year ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

2 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Mouse Colon using PureLink™ RNA Mini Kit from Thermo Fisher Scientific.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for PureLink™ RNA Mini Kit below.

Download PDF Download manufacturer protocol

Videos

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