RNA-Bee

RNA isolation / purification Tissue - Mouse Colon

Experiment

RNA isolation / purification Tissue - Mouse Colon

Product

RNA-Bee from Amsbio

Manufacturer

Amsbio

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Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- Isolation of RNA from a small amount of tissue (1-10 mg) can be accomplished by homogenizing the sample in 0.8 ml of RNA-Bee. - Adjust buffers to pH to 6.5 - 7.5 if they have have an acidic pH for better results.	- An additional wash with 75% ethanol improves 260/280 ratio

Upstream tips
- Isolation of RNA from a small amount of tissue (1-10 mg) can be accomplished by homogenizing the sample in 0.8 ml of RNA-Bee. - Adjust buffers to pH to 6.5 - 7.5 if they have have an acidic pH for better results.
Protocol tips
- An additional wash with 75% ethanol improves 260/280 ratio

Publication protocol

Total RNA was extracted from IECs or colon tissues with RNA bee according to the manufacturer's instructions (Tel-test, Friendswood, TX)

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Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

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Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Mouse Colon using RNA-Bee from Amsbio.

Paper title

Intestinal Epithelial Toll-Like Receptor 4 Signaling Affects Epithelial Function and Colonic Microbiota and Promotes a Risk for Transmissible Colitis

Full paper

Manufacturer protocol

Download the product protocol from Amsbio for RNA-Bee below.

Download manufacturer protocol

Videos

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