|- Isolation of RNA from a small amount of tissue (1-10 mg) can be accomplished by homogenizing the sample in 0.8 ml of RNA-Bee.
- Adjust buffers to pH to 6.5 - 7.5 if they have have an acidic pH for better results.
|- An additional wash with 75% ethanol improves 260/280 ratio
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2 years ago
Author: Paul G. Macon
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
3 years ago
Author: Aaron Stege
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
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