TRIzol Reagent
RNA isolation / purification Tissue - Mouse Esophagus
Protocol tips
| Protocol tips |
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| - For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it. |
Publication protocol
Total RNA was isolated with Trizol (Invitrogen), and 1 μg RNA was reverse transcribed with Quantitect (Qiagen). cDNA was then used as a template to perform qualitative PCR on a Perkin Elmer GeneAmp 9700 and qRT-PCR with an Applied Biosystems StepOnePlus machine. ΔΔCt method was used to determine relative transcript levels on qRT-PCRFull paper Login or join for free to view the full paper.
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Discussion
Discussion
4 years ago
Author:
Paul G. Macon
RNA isolation from tissue
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
Discussion
5 years ago
Author:
Aaron Stege
Problem in phase separation after using serum/plasma kit
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
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