RNeasy Fibrous Tissue Mini Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- This kit purifies RNA from up to 30 mg tissue		- If there is low RNA yield,Repeat RNA elution, incubate the RNeasy Mini spin column on the benchtop for 10 min with RNase-free water before centrifuging. - If there is low A260/A280 value use 10 mM Tris·Cl, pH 7.5, not RNase-free water, to dilute the sample before measuring purity. - If RNA does not perform well in downstream experiments this could be due to salt carryover dyuring elution step, so ensure that Buffer RPE is at 15–25°C.

Upstream tips
- This kit purifies RNA from up to 30 mg tissue
Downstream tips
- If there is low RNA yield,Repeat RNA elution, incubate the RNeasy Mini spin column on the benchtop for 10 min with RNase-free water before centrifuging. - If there is low A260/A280 value use 10 mM Tris·Cl, pH 7.5, not RNase-free water, to dilute the sample before measuring purity. - If RNA does not perform well in downstream experiments this could be due to salt carryover dyuring elution step, so ensure that Buffer RPE is at 15–25°C.

Publication protocol

Three mice of each group were sacrificed and the esophageal epitheliums were dissected. Total RNA was extracted from individual mouse esophagi with an RNeasy Fibrous Tissue Mini Kit (Qiagen, Valencia, CA). These RNA samples were checked for their quality using gel electrophoresis and their concentrations were measured using spectrophotometry. RNA quality (RIN>7) was further checked with Bioanalyzer (Agilent Technologies, Santa Clara, CA).

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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Fibrous Tissue Mini Kit below.

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