RNeasy Lipid Tissue Mini Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- If the RNA yield is low, repeat RNA elution, but incubate the RNeasy Mini spin column on the benchtop for 10 min with RNase-free water before centrifuging. - All centrifugation steps should be performed at 15–25°C except for phase separation

Protocol tips
- If the RNA yield is low, repeat RNA elution, but incubate the RNeasy Mini spin column on the benchtop for 10 min with RNase-free water before centrifuging. - All centrifugation steps should be performed at 15–25°C except for phase separation

Publication protocol

RNA preparation was carried out using the Lipid Tissue Mini Kit (Qiagen) following the manufacturer׳s instructions. Hippocampal tissue samples were lysed and homogenized using a handheld rotor-stator homogenizer (Tissue Ruptor, Qiagen) with disposable probes. All centrifugation steps were carried out at 10,000xg and RNA was finally eluted in 50 µl RNase free H2O. After preparation the quantity and quality of the eluted RNA was checked using Nanodrop (Thermo Scientific). Prior to RNA hybridization to the Microarray Chips the RNA quality was tested again using the Bioanaylzer 2100 (Agilent Technology)

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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Lipid Tissue Mini Kit below.

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