RNeasy Midi Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- This kit purifies RNA from up to 20–250 mg tissue		- If there is any DNA contamination in downstream applications incubate the RNeasy column for 5 min at room temperature with Buffer RW1 before centrifuging

Upstream tips
- This kit purifies RNA from up to 20–250 mg tissue
Downstream tips
- If there is any DNA contamination in downstream applications incubate the RNeasy column for 5 min at room temperature with Buffer RW1 before centrifuging

Publication protocol

Total RNA was isolated from murine ileum and liver (RNeasy Midi Kit, Qiagen, Hilden, Germany). RNA integrity was tested by RNA gel electrophoresis. cDNA was synthesized from total RNA using the iScript cDNA synthesis kit (BioRad, Hercules, CA, USA). Quantitative RT-PCR was performed using SYBR Green Supermix (BioRad) on an iCycler iQ system using diluted cDNA as template (primer sequences are provided in Table S1 in File S1). Expression levels were estimated using the comparative threshold cycle method. Cyclophilin was used as housekeeping gene, with similar expression levels in ileum and liver under all experimental conditions.

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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Midi Kit below.

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