NucleoSpin® RNA
RNA isolation / purification Tissue - Mouse Lymph node
Protocol tips
| Upstream tips |
|---|
| - Aliquot rDNase and store at -20 °C. |
| Protocol tips |
| - Dry the columns very well after the ethanol wash by adding an additional 2' >10000 rpm centrifuge with no buffer. |
Publication protocol
Total RNA from eyes and LNs was homogenized and extracted using TRIzol reagent (Qiagen) and NucleoSpin RNA II kit (Macherey-Nagel) or Qiagen RNeasy Micro kit. cDNA was reverse transcribed (Roche Applied Biosystem reagents) from 2 μg of RNA and analysed by quantitative PCR with SYBR green (Bio-Rad) with specific primers.Full paper Login or join for free to view the full paper.
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Discussion
Discussion
5 years ago
Author:
Paul G. Macon
RNA isolation from tissue
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
Discussion
5 years ago
Author:
Aaron Stege
Problem in phase separation after using serum/plasma kit
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
Papers
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Manufacturer protocol
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