SV Total RNA Isolation System
RNA isolation / purification Tissue - Mouse Muscle
Publication protocol
Total RNA was extracted from tissues (liver, adipose tissue, soleus muscle) using the SV Total RNA Isolation System kit (Promega Corporation) according to the manufacturer’s instructions. RNA concentrations were determined using a NanoDrop 2000 spectophotometer (Thermo Scientific)Full paper Login or join for free to view the full paper.
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Discussion
Discussion
4 years ago
Author:
Paul G. Macon
RNA isolation from tissue
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
Discussion
5 years ago
Author:
Aaron Stege
Problem in phase separation after using serum/plasma kit
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
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Manufacturer protocol
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