NucleoSpin® RNA Plus
RNA isolation / purification Tissue - Mouse Retina
Protocol tips
| Upstream tips |
|---|
| - This kit is designed for tissue samples of < 30 mg tissue. |
| Downstream tips |
| - For adsorption measurement, use 5 mM Tris pH 8.5 as diluent. |
Publication protocol
Total RNA was isolated from 1 × 105 BV2 cells or from individual mouse retinal extracts immediately after euthanasia using NucleoSpin® RNA Plus kit (Macherey-Nagel GmbH (available online: http://www.mn-net.com/)), following the manufacturers protocol.Full paper Login or join for free to view the full paper.
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Discussion
Discussion
4 years ago
Author:
Paul G. Macon
RNA isolation from tissue
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
Discussion
4 years ago
Author:
Aaron Stege
Problem in phase separation after using serum/plasma kit
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
Papers
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Manufacturer protocol
Download the product protocol from Macherey Nagel for NucleoSpin® RNA Plus below.
Download manufacturer protocol
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