NucleoSpin® RNA Plus

RNA isolation / purification Tissue - Mouse Retina

Experiment

RNA isolation / purification Tissue - Mouse Retina

Product

NucleoSpin® RNA Plus from Macherey Nagel

Manufacturer

Macherey Nagel

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Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- This kit is designed for tissue samples of < 30 mg tissue.		- For adsorption measurement, use 5 mM Tris pH 8.5 as diluent.

Upstream tips
- This kit is designed for tissue samples of < 30 mg tissue.
Downstream tips
- For adsorption measurement, use 5 mM Tris pH 8.5 as diluent.

Publication protocol

Total RNA was isolated from 1 × 105 BV2 cells or from individual mouse retinal extracts immediately after euthanasia using NucleoSpin® RNA Plus kit (Macherey-Nagel GmbH (available online: http://www.mn-net.com/)), following the manufacturers protocol.

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Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

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Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Mouse Retina using NucleoSpin® RNA Plus from Macherey Nagel.

Paper title

Concentrated Conditioned Media from Adipose Tissue Derived Mesenchymal Stem Cells Mitigates Visual Deficits and Retinal Inflammation Following Mild Traumatic Brain Injury

Full paper

Manufacturer protocol

Download the product protocol from Macherey Nagel for NucleoSpin® RNA Plus below.

Download manufacturer protocol

Videos

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