RNeasy Mini Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).	- Include DNAse treatment for 15-20min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C. - Use water to elute the RNA that is warmed to ~60`C.

Protocol tips
- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).
Downstream tips
- Include DNAse treatment for 15-20min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C. - Use water to elute the RNA that is warmed to ~60`C.

Publication protocol

Following fundus imaging and rhodamine B angiography, mice were sacrificed by decapitation. Six retinas from 6 mice were combined to one sample. Three replicate retinal tissue samples were obtained at each time point in each treatment group (laser treatment of all veins or equivalent laser treatment between the large vessels). Veins of one eye of each mouse were occluded while the other eye served as control receiving the same laser application scheme in retinal areas between the large vessels. RNA was isolated from the retinas with the RNeasy kit (Qiagen, Hilden, Germany).

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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Mini Kit below.

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