SV Total RNA Isolation System

RNA isolation / purification Tissue - Mouse Skin

Experiment
RNA isolation / purification Tissue - Mouse Skin
Product
SV Total RNA Isolation System from Promega
Manufacturer
Promega

Protocol tips

Protocol tips
- Do not freeze-thaw the DNase more than three times after rehydration

Publication protocol

On RNA purification, biopsies were removed from RNAlater-ICE and transferred to 175 μl of SV RNA lysis buffer added β-mercaptoethanol (SV Total RNA Isolation System; Promega, Madison, WI) and homogenized. RNA purification was completed according to the manufacturer’s instructions (SV Total RNA Isolation System; Promega). RNA was stored until further use at −80°C.

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Discussion

Discussion

2 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

2 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Mouse Skin using SV Total RNA Isolation System from Promega.

Paper title
Anti-inflammatory effect of a retrovirus-derived immunosuppressive peptide in mouse models
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Manufacturer protocol

Download the product protocol from Promega for SV Total RNA Isolation System below.

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Videos

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