TRIzol Reagent

RNA isolation / purification Tissue - Mouse Skull

Experiment
RNA isolation / purification Tissue - Mouse Skull
Product
TRIzol Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

RNA was isolated using the TRIzol┬« extraction method. The quality of 1┬ÁL of each sample was checked using an Agilent Bioanalyzer 2100 to determine the RIN (RNA Integrity) score using the Agilent RNA 6000 Nano chip and reagents. Samples with a RIN score >7 were retained and converted to cDNA by Illumina RNA seq kit for sequence library preparation using the Illumina TruSeq RNA Sample Prep Guide: Low Throughput (LT) Protocol. The final libraries were then sequenced on an Illumina HiSeq 2000 at the Vanderbilt University genomics core facility.

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Discussion

Discussion

2 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

3 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Mouse Skull using TRIzol Reagent from Thermo Fisher Scientific.

Paper title
Characterization of Distinct Classes of Differential Gene Expression in Osteoblast Cultures from Non-Syndromic Craniosynostosis Bone
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

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Videos

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