Lipofectamine® LTX Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Do not add antibiotics to the Transfection Medium

Protocol tips
Do not add antibiotics to the Transfection Medium

Publication protocol

Transfection of plasmid DNA
Transient transfection of human Bip promoter reporter plasmid containing firefly luciferase (2 µg of pBipPro‐Luc), in which the Bip promoter region was cloned into the pGL4.14 vector (Promega, Madison, WI, USA) as described previously 10, was performed using Lipofectamine LTX (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's protocol 11. The reporter assay using a luminometer was performed using 0.2 µg of pRL‐SV40 plasmid DNA (Promega) as an internal control by the Dual‐Glo Luciferase Assay System (Promega) as described previously 11. The same concentration of plasmid DNAs was used in all cell lines. Thapsigargin (Tg) was used for the induction of endoplasmic reticulum (ER) stress after transfection as described previously 11-13.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA transfection Mammalian cells - Immortalized cell lines HeLa using Lipofectamine® LTX Reagent from Thermo Fisher Scientific.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Lipofectamine® LTX Reagent below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing DNA transfection Mammalian cells - Immortalized cell lines HeLa using Lipofectamine® LTX Reagent from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.