PureLink™ RNA Mini Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- Depending on the material you want to isolate RNA from, please refer to the manual for additional reagents/lab equipment that are required	- DNase is not supplied with this kit, a DNase kit is available which is compatible with this kit

Protocol tips
- Depending on the material you want to isolate RNA from, please refer to the manual for additional reagents/lab equipment that are required
Downstream tips
- DNase is not supplied with this kit, a DNase kit is available which is compatible with this kit

Publication protocol

Ten milligrams of tissue mass was used to obtain high quality, DNA-free, RNA with Pure Link RNA Mini Kit (Ambion, Carlsbad, CA, U.S.A.) according to manufacturer’s instructions, to ensure high-quality DNAse solution was applied to each sample and traces of DNAse were eliminated by additional washing steps. NanoDrop 1000 spectrophotometer (PEQLAB Biotechnologie, Erlangen, Germany) was used to assess the concentration and purity of aqueous RNA samples as described [48,49]. In brief, the absorbance ratio 260/280 was calculated and values between 1.95 and 2.05 were regarded as pure RNA. In order to generate cDNA, 1 μg of high-quality RNA of each individual sample was transcribed into cDNA using thermostable RNAse inhibitor during reverse transcription

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for PureLink™ RNA Mini Kit below.

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