RNeasy Plus Universal Kits
RNA isolation / purification Tissue - Mouse Thymus
Protocol tips
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| - Kits should be stored dry at room temperature (15–25°C). - This kit purifies RNA from up to 50 mg tissue |
Publication protocol
After tissue harvesting, a fraction of the maternal liver as well as fetal liver and thymus were preserved in RNAlater (Ambion by Life Technologies GmbH) at −20°C. Tissue homogenization was performed using micropackaging vials with ceramic beads (1.4 mm) in a Precellys 24 Tissue Homogenizer (Peqlab, Erlangen, Germany). RNA isolation and DNA digestion were conducted by use of RNeasy Plus Universal Mini Kit (Qiagen, Hilden, Germany) and DNA-Free Kit (Applied Biosystems by Life Technologies GmbH), respectively. cDNA synthesis was performed with random primers (Invitrogen by Life Technologies GmbH). The concentration and purity of RNA and cDNA were assessed using NanoQuant (Tecan Group AG, Männedorf, Switzerland).Full paper Login or join for free to view the full paper.
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Discussion
Discussion
4 years ago
Author:
Paul G. Macon
RNA isolation from tissue
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
Discussion
5 years ago
Author:
Aaron Stege
Problem in phase separation after using serum/plasma kit
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
Papers
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Manufacturer protocol
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Videos
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