RNAqueous™ Total RNA Isolation Kit
RNA isolation / purification Tissue - Mouse Uterus
Publication protocol
For RNA isolation and gene expression studies, immediately homogenize one of the lanced endometriotic lesions (or ~20 μg uterine tissue) in lysis binding solution and store at -80°C for future RNA isolation with the RNAqueous kit (Ambion) or other method as desired.Full paper Login or join for free to view the full paper.
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Discussion
Discussion
4 years ago
Author:
Paul G. Macon
RNA isolation from tissue
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
Discussion
5 years ago
Author:
Aaron Stege
Problem in phase separation after using serum/plasma kit
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
Papers
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Manufacturer protocol
Download the product protocol from Thermo Fisher Scientific for RNAqueous™ Total RNA Isolation Kit below.
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