RNeasy Lipid Tissue Mini Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- If the RNA yield is low, repeat RNA elution, but incubate the RNeasy Mini spin column on the benchtop for 10 min with RNase-free water before centrifuging. - All centrifugation steps should be performed at 15–25°C except for phase separation.

Protocol tips
- If the RNA yield is low, repeat RNA elution, but incubate the RNeasy Mini spin column on the benchtop for 10 min with RNase-free water before centrifuging. - All centrifugation steps should be performed at 15–25°C except for phase separation.

Publication protocol

Total RNA was extracted from Sb35-, As35-, or P35-treated vascular endothelial cells that were transfected with or without the siRNAs by using RNeasy Lipid Tissue Mini kit (Qiagen, Valencia, CA, USA).

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Papers

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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Lipid Tissue Mini Kit below.

Download manufacturer protocol

Videos

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