RNeasy Lipid Tissue Mini Kit

RNA isolation / purification Cells - primary bovine aortic endothelial cells

Experiment
RNA isolation / purification Cells - primary bovine aortic endothelial cells
Product
RNeasy Lipid Tissue Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
- If the RNA yield is low, repeat RNA elution, but incubate the RNeasy Mini spin column on the benchtop for 10 min with RNase-free water before centrifuging.

- All centrifugation steps should be performed at 15–25°C except for phase separation.

Publication protocol

Total RNA was extracted from Sb35-, As35-, or P35-treated vascular endothelial cells that were transfected with or without the siRNAs by using RNeasy Lipid Tissue Mini kit (Qiagen, Valencia, CA, USA).

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Reviews

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary bovine aortic endothelial cells using RNeasy Lipid Tissue Mini Kit from Qiagen.

Paper title
Transcriptional Induction of Metallothionein by Tris(pentafluorophenyl)stibane in Cultured Bovine Aortic Endothelial Cells
Full paper
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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Lipid Tissue Mini Kit below.

Download PDF Download manufacturer protocol

Videos

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