Protocol tips
Upstream tips |
Protocol tips |
Downstream tips |
|
- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer). |
- Include DNAse treatment for 15-20min.
- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.
- Use water to elute the RNA that is warmed to ~60`C.
|
Protocol tips |
- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer). |
Downstream tips |
- Include DNAse treatment for 15-20min.
- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.
- Use water to elute the RNA that is warmed to ~60`C.
|
Publication protocol
Total RNA was collected and purified from BTV-infected and uninfected (control) bPAEC and bMDM using the RNAeasy kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions, and cDNA was synthesized using a reverse transcription kit (Qiagen, Valencia, CA).
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Papers
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Paper title
Bluetongue virus infection activates bovine monocyte-derived macrophages and pulmonary artery endothelial cells
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