TRIzol Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

RNA was isolated from bPAEC, as previously described (5, 7). Briefly, Trizol (Life Technologies) was added to the cells and incubated for 5 min at room temperature. Chloroform (0.2 ml) was added, and the tubes were shaken for 15 s and then incubated at room temperature for 3 min. The mixture was centrifuged at 12,000 g for 15 min at 4°C. The supernatant was transferred to a fresh tube. Isopropyl alcohol (0.5 ml) was added, and the mixture incubated at room temperature for 10 min, then centrifuged at 12,000 g for 15 min at 4°C. The supernatant was discarded, and the pellet was washed with 75% ethanol and centrifuged at 7,500 g for 5 min at 4°C. The supernatant was discarded, and the pellet partially dried, dissolved in RNase free water, and stored at −80°C.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

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