TRIzol Reagent

RNA isolation / purification Cells - primary bovine pulmonary artery endothelial cells

Experiment
RNA isolation / purification Cells - primary bovine pulmonary artery endothelial cells
Product
TRIzol Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

RNA was isolated from bPAEC, as previously described (5, 7). Briefly, Trizol (Life Technologies) was added to the cells and incubated for 5 min at room temperature. Chloroform (0.2 ml) was added, and the tubes were shaken for 15 s and then incubated at room temperature for 3 min. The mixture was centrifuged at 12,000 g for 15 min at 4°C. The supernatant was transferred to a fresh tube. Isopropyl alcohol (0.5 ml) was added, and the mixture incubated at room temperature for 10 min, then centrifuged at 12,000 g for 15 min at 4°C. The supernatant was discarded, and the pellet was washed with 75% ethanol and centrifuged at 7,500 g for 5 min at 4°C. The supernatant was discarded, and the pellet partially dried, dissolved in RNase free water, and stored at −80°C.

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary bovine pulmonary artery endothelial cells using TRIzol Reagent from Thermo Fisher Scientific.

Paper title
Nitric oxide suppression of cellular proliferation depends on cationic amino acid transporter activity in cytokine-stimulated pulmonary endothelial cells
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

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