RNeasy Mini Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).	- Include DNAse treatment for 15-20min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C. - Use water to elute the RNA that is warmed to ~60`C.

Protocol tips
- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).
Downstream tips
- Include DNAse treatment for 15-20min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C. - Use water to elute the RNA that is warmed to ~60`C.

Publication protocol

Total RNA from cells was obtained using the RNeasy kit (Qiagen, Hilden, Germany) following the manufacturer's instructions treated with the DNAse 1, amplification grade (Sigma, St Louis, MO), to eliminate the genome DNA contamination and quantified using the NanoDrop ND-1000 Spectrophotometer (Nano-Drop Technologies, Wilmington, DE). Absorbance of the RNA samples was quantified at 260 and 280 nm, and the 260/280 ratio was calculated. The samples showed a 260/280 ratio ≥1.9, which was assumed as an indicator of RNA purity.

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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Mini Kit below.

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