NucleoSpin® miRNA

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- For quantitative RNA purification from starting material less than 10^6cells, it is advantageous to add 10 μg of Carrier RNA) before binding of small RNA to improve RNA binding.

Upstream tips
- For quantitative RNA purification from starting material less than 10^6cells, it is advantageous to add 10 μg of Carrier RNA) before binding of small RNA to improve RNA binding.

Publication protocol

Total RNA was extracted from the cells by using NucleoSpin miRNA (MACHEREY-NAGEL, Düren, Deutschland) according to the standard protocol. RNA concentration and purity were assessed by UV spectrophotometry. RNA integrity was checked by formaldehyde gel electrophoresis.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary canine aortic endothelial cells using NucleoSpin® miRNA from Macherey Nagel.

Manufacturer protocol

Download the product protocol from Macherey Nagel for NucleoSpin® miRNA below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Cells - primary canine aortic endothelial cells using NucleoSpin® miRNA from Macherey Nagel. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.