NucleoSpin® miRNA

RNA isolation / purification Cells - primary canine aortic endothelial cells

Experiment
RNA isolation / purification Cells - primary canine aortic endothelial cells
Product
NucleoSpin® miRNA from Macherey Nagel
Manufacturer
Macherey Nagel

Protocol tips

Upstream tips
- For quantitative RNA purification from starting material less than 10^6cells, it is advantageous to add 10 μg of Carrier RNA) before binding of small RNA to improve RNA binding.

Publication protocol

Total RNA was extracted from the cells by using NucleoSpin miRNA (MACHEREY-NAGEL, Düren, Deutschland) according to the standard protocol. RNA concentration and purity were assessed by UV spectrophotometry. RNA integrity was checked by formaldehyde gel electrophoresis.

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Reviews

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Discussion

Discussion

2 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary canine aortic endothelial cells using NucleoSpin® miRNA from Macherey Nagel.

Paper title
MicroRNA-214 Promotes Apoptosis in Canine Hemangiosarcoma by Targeting the COP1-p53 Axis
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Manufacturer protocol

Download the product protocol from Macherey Nagel for NucleoSpin® miRNA below.

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Videos

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