TRIzol Reagent

RNA isolation / purification Cells - primary canine marrow stromal cells

Experiment
RNA isolation / purification Cells - primary canine marrow stromal cells
Product
TRIzol Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

RNA was extracted from MSC, primary skin fibroblast, peripheral blood mononuclear cells (PBMC), and fresh marrow cells using TRIzol (Invitrogen, Carlsbad CA, USA) and chloroform. Isopropanol and 75% ethanol were used to precipitate and resuspend the RNA. The RNA was dissolved in 100 μL of water. cDNA was prepared using standard methods and reagents (Invitrogen).

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Reviews

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary canine marrow stromal cells using TRIzol Reagent from Thermo Fisher Scientific.

Paper title
Canine Bone Marrow Derived Mesenchymal Stromal Cells Suppress Allo-Reactive Lymphocyte Proliferation in Vitro but Fail to Enhance Engraftment in Canine Bone Marrow Transplantation
Full paper
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

Download PDF Download manufacturer protocol

Videos

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