TRIzol Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

RNA was extracted from MSC, primary skin fibroblast, peripheral blood mononuclear cells (PBMC), and fresh marrow cells using TRIzol (Invitrogen, Carlsbad CA, USA) and chloroform. Isopropanol and 75% ethanol were used to precipitate and resuspend the RNA. The RNA was dissolved in 100 μL of water. cDNA was prepared using standard methods and reagents (Invitrogen).

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

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