High Pure RNA Isolation Kit

RNA isolation / purification Cells - primary human aortic smooth muscle cells

Experiment
RNA isolation / purification Cells - primary human aortic smooth muscle cells
Product
High Pure RNA Isolation Kit from Roche Lifesciences
Manufacturer
Roche Lifesciences

Protocol tips

Upstream tips
- To decrease RNase activity,process starting material immeditely or store at 80C.

- To increase nucleic yield or purity store all buffer at +15C to +25C.
Protocol tips
- To decrease RNase activity,use eluted RNA directly in downstream procedure or store at -80C immediately.

Publication protocol

Total RNA was extracted from the cultured cells using a High Pure RNA Isolation Kit (RNAiso Plus; Takara Bio, Shiga, Japan). The purity and quantity of the RNA preparations were determined by measuring the optical densities of the solutions at 260 and 280 nm.

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Reviews

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary human aortic smooth muscle cells using High Pure RNA Isolation Kit from Roche Lifesciences.

Paper title
-Cresyl sulfate, a uremic toxin, causes vascular endothelial and smooth muscle cell damages by inducing oxidative stress
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Manufacturer protocol

Download the product protocol from Roche Lifesciences for High Pure RNA Isolation Kit below.

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Videos

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