TRI Reagent® Sigma

RNA isolation / purification Cells - primary human bronchial epithelial cells

Experiment
RNA isolation / purification Cells - primary human bronchial epithelial cells
Product
TRI Reagent® Sigma from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Upstream tips
- 1-Bromo-3-chloropropane is less toxic than chloroform and its use for phase separation decreases the possibility of contaminating RNA with DNA.

- The chloroform used for phase separation should not contain isoamyl alcohol or other additives.

Publication protocol

After the treatment, the cells were washed with PBS-solution and the total RNA was extracted using TRI-reagent as described previously (Storvik et al. 2011). The extracted RNA was stored at -80 oC before analysis. Three replicates were used for further study.

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary human bronchial epithelial cells using TRI Reagent® Sigma from Sigma-Aldrich.

Paper title
Molecular targets of chloropicrin in human airway epithelial cells.
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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for TRI Reagent® Sigma below.

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Videos

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