TRI Reagent® Sigma

Protocol tips

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- 1-Bromo-3-chloropropane is less toxic than chloroform and its use for phase separation decreases the possibility of contaminating RNA with DNA. - The chloroform used for phase separation should not contain isoamyl alcohol or other additives.

Upstream tips
- 1-Bromo-3-chloropropane is less toxic than chloroform and its use for phase separation decreases the possibility of contaminating RNA with DNA. - The chloroform used for phase separation should not contain isoamyl alcohol or other additives.

Publication protocol

RNA was extracted from fish and cell samples using Tri-Reagent (SIGMA-ALDRICH) according to manufacturer’s instruction.

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for TRI Reagent® Sigma below.

Download manufacturer protocol

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