ICAfectin®442 siRNA transfection

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Do not include serum and antibiotics during the formation of the ICAFectin 442/siRNA complexes.

Protocol tips
Do not include serum and antibiotics during the formation of the ICAFectin 442/siRNA complexes.

Publication protocol

Transient transfections of HeLa cells and COS-7 with siRNA and/or plasmids were performed using ICAFectin442 (Eurogentec), JetPrime (Polyplus), or FuGENE 6 (Roche) following the manufacturer's instructions. HEK293T cells were transfected with the calcium phosphate transfection method.
Stable transfection of HeLa cells with shRNA or plasmids was performed using FuGENE 6. HeLa clones were obtained by limiting dilutions. For generation of retroviral supernatants, pMSCV-puro vector (encoding different Optn constructs or control vector) was transfected into Plat-A packaging cell line using FuGENE 6. High titers of recombinant viruses were obtained 48 hr after transfection and used to infect Optn-depleted HeLa cells.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA transfection Mammalian cells - Immortalized cell lines COS7 using ICAfectin®442 siRNA transfection from Incellart.

Manufacturer protocol

Download the product protocol from Incellart for ICAfectin®442 siRNA transfection below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing DNA transfection Mammalian cells - Immortalized cell lines COS7 using ICAfectin®442 siRNA transfection from Incellart. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.