TRIzol Reagent

RNA isolation / purification Cells - primary human coronary artery smooth muscle cells

Experiment
RNA isolation / purification Cells - primary human coronary artery smooth muscle cells
Product
TRIzol Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

Briefly, total HCAEC RNA of each time point was extracted using TRIzol® reagent (Cat. No. 15596-026, Invitrogen) with additional purification using the RNeasy MiniElute Cleanup Kit (Cat. No. 74204, Qiagen, Inc., Valencia, CA). Total RNA (5 μg) from each sample was converted into double-stranded cDNA using SuperScript Choice system (Cat. No. 11917-010, Invitrogen).

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary human coronary artery smooth muscle cells using TRIzol Reagent from Thermo Fisher Scientific.

Paper title
Temporal Gene Expression Analysis of Human Coronary Artery Endothelial Cells Treated With Simvastatin
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

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