TRIzol Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

Briefly, total HCAEC RNA of each time point was extracted using TRIzol® reagent (Cat. No. 15596-026, Invitrogen) with additional purification using the RNeasy MiniElute Cleanup Kit (Cat. No. 74204, Qiagen, Inc., Valencia, CA). Total RNA (5 μg) from each sample was converted into double-stranded cDNA using SuperScript Choice system (Cat. No. 11917-010, Invitrogen).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary human coronary artery smooth muscle cells using TRIzol Reagent from Thermo Fisher Scientific.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Cells - primary human coronary artery smooth muscle cells using TRIzol Reagent from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.