SV Total RNA Isolation System

RNA isolation / purification Cells - primary human coronary artery smooth muscle cells

Experiment
RNA isolation / purification Cells - primary human coronary artery smooth muscle cells
Product
SV Total RNA Isolation System from Promega
Manufacturer
Promega

Protocol tips

Protocol tips
- Do not freeze-thaw the DNase more than three times after rehydration.

- For better lysis, pipet the RNA Lysis Buffer over the bottom of the well.

Publication protocol

Before RT-PCR, total RNA from endothelial cell cultures was isolated using Total RNA Isolation System (Promega, USA) following manufacturer's instructions. The purity and amount of RNA were determined by measuring the OD at a ratio of 260 to 280 nm. 1 μg of total RNA were transcribed into DNA by Reverse Transcription System (Promega, USA) and PCR was performed for β-actin, IL-6, and TF (Table 1). β-actin was used as a control for normalization.

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Reviews

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary human coronary artery smooth muscle cells using SV Total RNA Isolation System from Promega.

Paper title
Increased Responsiveness of Human Coronary Artery Endothelial Cells in Inflammation and Coagulation
Full paper
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Manufacturer protocol

Download the product protocol from Promega for SV Total RNA Isolation System below.

Download PDF Download manufacturer protocol

Videos

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