SV Total RNA Isolation System

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- Do not freeze-thaw the DNase more than three times after rehydration. - For better lysis, pipet the RNA Lysis Buffer over the bottom of the well.

Protocol tips
- Do not freeze-thaw the DNase more than three times after rehydration. - For better lysis, pipet the RNA Lysis Buffer over the bottom of the well.

Publication protocol

Before RT-PCR, total RNA from endothelial cell cultures was isolated using Total RNA Isolation System (Promega, USA) following manufacturer's instructions. The purity and amount of RNA were determined by measuring the OD at a ratio of 260 to 280 nm. 1 μg of total RNA were transcribed into DNA by Reverse Transcription System (Promega, USA) and PCR was performed for β-actin, IL-6, and TF (Table 1). β-actin was used as a control for normalization.

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Manufacturer protocol

Download the product protocol from Promega for SV Total RNA Isolation System below.

Download manufacturer protocol

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