High Pure RNA Isolation Kit

RNA isolation / purification Cells - primary human dermal fibroblasts

Experiment
RNA isolation / purification Cells - primary human dermal fibroblasts
Product
High Pure RNA Isolation Kit from Roche Lifesciences
Manufacturer
Roche Lifesciences

Protocol tips

Upstream tips
- To decrease RNase activity,process starting material immeditely or store at 80C.

- To increase nucleic yield or purity store all buffer at +15C to +25C.
Protocol tips
- To decrease RNase activity,use eluted RNA directly in downstream procedure or store at -80C immediately.

Publication protocol

Total RNA from HDF cultured for 24 h was extracted by means of the High Pure RNA Isolation Kit (Roche Applied Science, Mannheim, Germany) according to the manufacturer’s protocol. First-strand cDNA was synthesized by using PrimeScript RT reagent kit (Perfect Real Time; Takara Bio).

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Reviews

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary human dermal fibroblasts using High Pure RNA Isolation Kit from Roche Lifesciences.

Paper title
Hydrolyzed eggshell membrane immobilized on phosphorylcholine polymer supplies extracellular matrix environment for human dermal fibroblasts
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Manufacturer protocol

Download the product protocol from Roche Lifesciences for High Pure RNA Isolation Kit below.

Download PDF Download manufacturer protocol

Videos

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