High Pure RNA Isolation Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- To decrease RNase activity,process starting material immeditely or store at 80C. - To increase nucleic yield or purity store all buffer at +15C to +25C.	- To decrease RNase activity,use eluted RNA directly in downstream procedure or store at -80C immediately.

Upstream tips
- To decrease RNase activity,process starting material immeditely or store at 80C. - To increase nucleic yield or purity store all buffer at +15C to +25C.
Protocol tips
- To decrease RNase activity,use eluted RNA directly in downstream procedure or store at -80C immediately.

Publication protocol

Total RNA from HDF cultured for 24 h was extracted by means of the High Pure RNA Isolation Kit (Roche Applied Science, Mannheim, Germany) according to the manufacturer’s protocol. First-strand cDNA was synthesized by using PrimeScript RT reagent kit (Perfect Real Time; Takara Bio).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary human dermal fibroblasts using High Pure RNA Isolation Kit from Roche Lifesciences.

Manufacturer protocol

Download the product protocol from Roche Lifesciences for High Pure RNA Isolation Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Cells - primary human dermal fibroblasts using High Pure RNA Isolation Kit from Roche Lifesciences. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.