TRIzol Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

Total RNA was extracted by using TRIzol reagent (Invitrogen), quantified by densitometric analysis at 260 nm, and analyzed by real-time reverse transcription-PCR (RT-PCR) using ORF 73, ORF 50, and K8 primers as described previously (3, 20). PCR was performed using an ABI Prism 7500 real-time PCR system utilizing TaqMan EZ RT-PCR Core reagents (Applied Biosystems).

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

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