TRI Reagent® MRC

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- 1-Bromo-3-chloropropane is less toxic than chloroform and its use for phase separation decreases the possibility of contaminating RNA with DNA. - The chloroform used for phase separation should not contain isoamyl alcohol or other additives.

Upstream tips
- 1-Bromo-3-chloropropane is less toxic than chloroform and its use for phase separation decreases the possibility of contaminating RNA with DNA. - The chloroform used for phase separation should not contain isoamyl alcohol or other additives.

Publication protocol

RNA extraction was performed using Tri Reagent (Molecular Research Center). Two micrograms of total RNA were reverse transcribed following the instructions for the high-capacity cDNA RT (Thermo Fisher) protocol, and samples were stored at −70°C ().

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Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary human dermal microvascular endothelial cells using TRI Reagent® MRC from Molecular Research Center, Inc..

Manufacturer protocol

Download the product protocol from Molecular Research Center, Inc. for TRI Reagent® MRC below.

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Videos

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