AllPrep RNA/Protein Kit

RNA isolation / purification Cells - primary human epidermal keratinocytes

Experiment

RNA isolation / purification Cells - primary human epidermal keratinocytes

Product

AllPrep RNA/Protein Kit from Qiagen

Manufacturer

Qiagen

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Protocol tips

Upstream tips	Protocol tips	Downstream tips
	mRNA and protein was extracted from monolayers of HEKn and HaCaT cells using AllPrep Protein/RNA Isolation Kit according to manufacturer’s protocol (QIAGEN GmBH, Hilden Germany), quantified using the NanoDrop Lite Spectrophotometer (ThermoScientific, Wilmington, USA) and expression of differentiation markers Keratin 14 (K14), Keratin 10 (K10) and Involucrin (Inv) assessed by qRT-PCR and Western blot.

Protocol tips
mRNA and protein was extracted from monolayers of HEKn and HaCaT cells using AllPrep Protein/RNA Isolation Kit according to manufacturer’s protocol (QIAGEN GmBH, Hilden Germany), quantified using the NanoDrop Lite Spectrophotometer (ThermoScientific, Wilmington, USA) and expression of differentiation markers Keratin 14 (K14), Keratin 10 (K10) and Involucrin (Inv) assessed by qRT-PCR and Western blot.

Protocol tips

mRNA and protein was extracted from monolayers of HEKn and HaCaT cells using AllPrep Protein/RNA Isolation Kit according to manufacturer’s protocol (QIAGEN GmBH, Hilden Germany), quantified using the NanoDrop Lite Spectrophotometer (ThermoScientific, Wilmington, USA) and expression of differentiation markers Keratin 14 (K14), Keratin 10 (K10) and Involucrin (Inv) assessed by qRT-PCR and Western blot.

Publication protocol

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Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary human epidermal keratinocytes using AllPrep RNA/Protein Kit from Qiagen.

Paper title

Combination of Low Calcium with Y-27632 Rock Inhibitor Increases the Proliferative Capacity, Expansion Potential and Lifespan of Primary Human Keratinocytes while Retaining Their Capacity to Differentiate into Stratified Epidermis in a 3D Skin Model

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Manufacturer protocol

Download the product protocol from Qiagen for AllPrep RNA/Protein Kit below.

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Videos

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