AllPrep RNA/Protein Kit

RNA isolation / purification Cells - primary human epidermal keratinocytes

Experiment
RNA isolation / purification Cells - primary human epidermal keratinocytes
Product
AllPrep RNA/Protein Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
mRNA and protein was extracted from monolayers of HEKn and HaCaT cells using AllPrep Protein/RNA Isolation Kit according to manufacturer’s protocol (QIAGEN GmBH, Hilden Germany), quantified using the NanoDrop Lite Spectrophotometer (ThermoScientific, Wilmington, USA) and expression of differentiation markers Keratin 14 (K14), Keratin 10 (K10) and Involucrin (Inv) assessed by qRT-PCR and Western blot.

Publication protocol

mRNA and protein was extracted from monolayers of HEKn and HaCaT cells using AllPrep Protein/RNA Isolation Kit according to manufacturer’s protocol (QIAGEN GmBH, Hilden Germany), quantified using the NanoDrop Lite Spectrophotometer (ThermoScientific, Wilmington, USA) and expression of differentiation markers Keratin 14 (K14), Keratin 10 (K10) and Involucrin (Inv) assessed by qRT-PCR and Western blot.

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary human epidermal keratinocytes using AllPrep RNA/Protein Kit from Qiagen.

Paper title
Combination of Low Calcium with Y-27632 Rock Inhibitor Increases the Proliferative Capacity, Expansion Potential and Lifespan of Primary Human Keratinocytes while Retaining Their Capacity to Differentiate into Stratified Epidermis in a 3D Skin Model
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Manufacturer protocol

Download the product protocol from Qiagen for AllPrep RNA/Protein Kit below.

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Videos

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