TRIzol Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

RNA was extracted using TRIzol (Invitrogen), and selected genes (Figs ​(Figs1d1d and e, ​e,2d2d and e and ​and4b4b were amplified by quantitative real-time reverse transcriptase PCR (qRT-PCR) with SYBR green (Qiagen, Germantown, Maryland, USA) on a FAST Model 7900HT (Applied Biosystems, Foster City, California, USA) as previously published 31. Primer sequences are given in Table ​Table1.1. Data were analyzed on SDS 7900HT software v2.2.2 (Applied Biosystems, Grand Island, New York, USA) using the comparative threshold cycle (Ct) method (An external file that holds a picture, illustration, etc.
Object name is cmr-25-376-i001.jpg) for calculating fold changes and SDs 32.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary human epidermal melanocytes using TRIzol Reagent from Thermo Fisher Scientific.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Cells - primary human epidermal melanocytes using TRIzol Reagent from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.