TRIzol Reagent

RNA isolation / purification Cells - primary human epidermal melanocytes

Experiment
RNA isolation / purification Cells - primary human epidermal melanocytes
Product
TRIzol Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

RNA was extracted using TRIzol (Invitrogen), and selected genes (Figs ​(Figs1d1d and e, ​e,2d2d and e and ​and4b4b were amplified by quantitative real-time reverse transcriptase PCR (qRT-PCR) with SYBR green (Qiagen, Germantown, Maryland, USA) on a FAST Model 7900HT (Applied Biosystems, Foster City, California, USA) as previously published 31. Primer sequences are given in Table ​Table1.1. Data were analyzed on SDS 7900HT software v2.2.2 (Applied Biosystems, Grand Island, New York, USA) using the comparative threshold cycle (Ct) method (An external file that holds a picture, illustration, etc.
Object name is cmr-25-376-i001.jpg) for calculating fold changes and SDs 32.

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary human epidermal melanocytes using TRIzol Reagent from Thermo Fisher Scientific.

Paper title
A combination of high dose rate (10X FFF/2400 MU/min/10 MV X-rays) and total low dose (0.5 Gy) induces a higher rate of apoptosis in melanoma cells and superior preservation of normal melanocytes
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

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Videos

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