TRIzol Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

RNA isolation was carried out using TRIzol™ reagent. After DNase1, cDNA was synthesized using ImProm‐II™ Reverse transcription system (Promega, USA). mRNA expression analysis was performed using Brilliant III ultra‐fast SYBR®Green qPCR master mix (Agilent Technologies, USA). Expression levels are relative to HPRT (HPRT‐gene). Primers sequences are listed in Appendix Table S1.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

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