TRIzol Reagent

RNA isolation / purification Cells - primary human lung fibroblasts

Experiment
RNA isolation / purification Cells - primary human lung fibroblasts
Product
TRIzol Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

RNA isolation was carried out using TRIzol™ reagent. After DNase1, cDNA was synthesized using ImProm‐II™ Reverse transcription system (Promega, USA). mRNA expression analysis was performed using Brilliant III ultra‐fast SYBR®Green qPCR master mix (Agilent Technologies, USA). Expression levels are relative to HPRT (HPRT‐gene). Primers sequences are listed in Appendix Table S1.

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary human lung fibroblasts using TRIzol Reagent from Thermo Fisher Scientific.

Paper title
FoxO3 an important player in fibrogenesis and therapeutic target for idiopathic pulmonary fibrosis
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

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