RNAqueous™ Total RNA Isolation Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- This kit can be used for the isolation of RNA from 1 x 10^2–1 x 10^7

Upstream tips
- This kit can be used for the isolation of RNA from 1 x 10^2–1 x 10^7

Publication protocol

Total RNA was isolated from HLF cells co-cultured with BEC’s grown at an ALI. Three wells from each experimental condition were harvested and pooled to isolate RNA using the RNAqueous kit for total RNA purification from Ambion®-Applied Biosystems (Austin, TX). RNA concentration and integrity were determined using the Agilent® 2100 Bioanalyzer system and Agilent® RNA 6000 Nano Chips (Agilent® Technologies, Foster City, CA). RNA samples (1 μg) with a RNA integrity number (RIN) ≥ 8 were reverse transcribed with MMLV reverse transcriptase with a combination of random hexamers and oligo-dTs using the SuperScript® VILO cDNA Synthesis Kit (Life Technologies, Grand Island, NY).

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for RNAqueous™ Total RNA Isolation Kit below.

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