RNAqueous™ Total RNA Isolation Kit

RNA isolation / purification Cells - primary human lung fibroblasts

Experiment
RNA isolation / purification Cells - primary human lung fibroblasts
Product
RNAqueous™ Total RNA Isolation Kit from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Upstream tips
- This kit can be used for the isolation of RNA from 1 x 10^2–1 x 10^7

Publication protocol

Total RNA was isolated from HLF cells co-cultured with BEC’s grown at an ALI. Three wells from each experimental condition were harvested and pooled to isolate RNA using the RNAqueous kit for total RNA purification from Ambion®-Applied Biosystems (Austin, TX). RNA concentration and integrity were determined using the Agilent® 2100 Bioanalyzer system and Agilent® RNA 6000 Nano Chips (Agilent® Technologies, Foster City, CA). RNA samples (1 μg) with a RNA integrity number (RIN) ≥ 8 were reverse transcribed with MMLV reverse transcriptase with a combination of random hexamers and oligo-dTs using the SuperScript® VILO cDNA Synthesis Kit (Life Technologies, Grand Island, NY).

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Reviews

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary human lung fibroblasts using RNAqueous™ Total RNA Isolation Kit from Thermo Fisher Scientific.

Paper title
Fibroblast-myofibroblast transition is differentially regulated by bronchial epithelial cells from asthmatic children
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for RNAqueous™ Total RNA Isolation Kit below.

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Videos

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