RNeasy Plus Micro Kit

RNA isolation / purification Cells - primary human lung microvascular endothelial cells

Experiment
RNA isolation / purification Cells - primary human lung microvascular endothelial cells
Product
RNeasy Plus Micro Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Upstream tips
- When processing <500 cells or <2 µg tissue, carrier RNA may be added to the lysate.

- Foaming can be reduced by adding Reagent DX at a final concentration of 0.5% (v/v) before disruption and homogenization.

- Add 4 volumes of ethanol (96–100%) to Buffer RPE for a working solution.
before homogenization

Publication protocol

Total cellular RNA was extracted with using RNeasy Plus Micro Kit from Qiagen (Valencia, CA) and reverse transcribed using random hexamer primers (High-Capacity cDNA Reverse Transcription Kit from Applied Biosystems, Carlsbad, CA). Real-time qPCR was performed using human IKBα and GAPDH TaqMan probes (Applied Biosystems) using StepOnePlus Real-Time PCR System (Applied Biosystems). All reactions were performed in triplicate; actin or GAPDH were used as internal standards.

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Reviews

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary human lung microvascular endothelial cells using RNeasy Plus Micro Kit from Qiagen.

Paper title
Hsp90 inhibition suppresses NF-κB transcriptional activation via Sirt-2 in human lung microvascular endothelial cells
Full paper
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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Plus Micro Kit below.

Download PDF Download manufacturer protocol

Videos

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