RNeasy Plus Micro Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- When processing <500 cells or <2 µg tissue, carrier RNA may be added to the lysate. - Foaming can be reduced by adding Reagent DX at a final concentration of 0.5% (v/v) before disruption and homogenization. - Add 4 volumes of ethanol (96–100%) to Buffer RPE for a working solution. before homogenization

Upstream tips
- When processing <500 cells or <2 µg tissue, carrier RNA may be added to the lysate. - Foaming can be reduced by adding Reagent DX at a final concentration of 0.5% (v/v) before disruption and homogenization. - Add 4 volumes of ethanol (96–100%) to Buffer RPE for a working solution. before homogenization

Publication protocol

Total cellular RNA was extracted with using RNeasy Plus Micro Kit from Qiagen (Valencia, CA) and reverse transcribed using random hexamer primers (High-Capacity cDNA Reverse Transcription Kit from Applied Biosystems, Carlsbad, CA). Real-time qPCR was performed using human IKBα and GAPDH TaqMan probes (Applied Biosystems) using StepOnePlus Real-Time PCR System (Applied Biosystems). All reactions were performed in triplicate; actin or GAPDH were used as internal standards.

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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Plus Micro Kit below.

Download manufacturer protocol

Videos

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