RNeasy Mini Kit

RNA isolation / purification Cells - primary human lung microvascular endothelial cells

Experiment
RNA isolation / purification Cells - primary human lung microvascular endothelial cells
Product
RNeasy Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).
Downstream tips
- Include DNAse treatment for 15-20min.

- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.

- Use water to elute the RNA that is warmed to ~60`C.

Publication protocol

Total RNA was isolated from either MSC MVs, HLMVECs or HLMVECs treated with (a) cytomix, (b) cytomix with MSC MVs, and (c) cytomix with MSC MVs pretreated with a scrabbled siRNA or Ang1 siRNA (90 μl/ml medium) using RNeasy Mini Kit (QIAGENSciences, Redwood City, CA, http://www.qiagen.com). The quality of the RNA was assessed with the NanoDrop ND‐1000 UV‐Vis Spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA); 260/280 and 260/230 nm absorbance ratios of 1.8–2.2 suggested a pure RNA sample.

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Reviews

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary human lung microvascular endothelial cells using RNeasy Mini Kit from Qiagen.

Paper title
Mesenchymal Stem Cell Microvesicles Restore Protein Permeability Across Primary Cultures of Injured Human Lung Microvascular Endothelial Cells
Full paper
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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Mini Kit below.

Download PDF Download manufacturer protocol

Videos

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