RNeasy Mini Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).	- Include DNAse treatment for 15-20min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C. - Use water to elute the RNA that is warmed to ~60`C.

Protocol tips
- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).
Downstream tips
- Include DNAse treatment for 15-20min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C. - Use water to elute the RNA that is warmed to ~60`C.

Publication protocol

Total RNA was isolated from either MSC MVs, HLMVECs or HLMVECs treated with (a) cytomix, (b) cytomix with MSC MVs, and (c) cytomix with MSC MVs pretreated with a scrabbled siRNA or Ang1 siRNA (90 μl/ml medium) using RNeasy Mini Kit (QIAGENSciences, Redwood City, CA, http://www.qiagen.com). The quality of the RNA was assessed with the NanoDrop ND‐1000 UV‐Vis Spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA); 260/280 and 260/230 nm absorbance ratios of 1.8–2.2 suggested a pure RNA sample.

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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Mini Kit below.

Download manufacturer protocol

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