RNeasy Mini Kit

RNA isolation / purification Cells - primary human marrow stromal cells

Experiment
RNA isolation / purification Cells - primary human marrow stromal cells
Product
RNeasy Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).
Downstream tips
- Include DNAse treatment for 15-20min.

- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.

- Use water to elute the RNA that is warmed to ~60`C.

Publication protocol

Total RNA was extracted from low passage, subconfluent cultures of hMSC from 3 individual donors and from peripheral blood mononuclear cells (PBMC) using the RNeasy Mini Kit (Qiagen; Valencia CA). A total of 500 ng of cellular RNA was used for cDNA synthesis using Bio-Rad iScript cDNA synthesis kit (Bio-Rad, Hercules, CA).

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Reviews

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary human marrow stromal cells using RNeasy Mini Kit from Qiagen.

Paper title
Rapidly Self-Renewing Human Multipotent Marrow Stromal Cells (hMSC) Express Sialyl Lewis X and Actively Adhere to Arterial Endothelium in a Chick Embryo Model System
Full paper
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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Mini Kit below.

Download PDF Download manufacturer protocol

Videos

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