RNeasy Mini Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).	- Include DNAse treatment for 15-20min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C. - Use water to elute the RNA that is warmed to ~60`C.

Protocol tips
- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).
Downstream tips
- Include DNAse treatment for 15-20min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C. - Use water to elute the RNA that is warmed to ~60`C.

Publication protocol

Total RNA was extracted from low passage, subconfluent cultures of hMSC from 3 individual donors and from peripheral blood mononuclear cells (PBMC) using the RNeasy Mini Kit (Qiagen; Valencia CA). A total of 500 ng of cellular RNA was used for cDNA synthesis using Bio-Rad iScript cDNA synthesis kit (Bio-Rad, Hercules, CA).

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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Mini Kit below.

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