mirVana™ miRNA Isolation Kit, with phenol

RNA isolation / purification Cells - primary human osteoblasts

Experiment
RNA isolation / purification Cells - primary human osteoblasts
Product
mirVana™ miRNA Isolation Kit, with phenol from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- For RNA to dissolve better and to yeild high levels, preheat elution buffer at 95C.

- Addition of β-mercaptoethanol in lysis buffer can be skipped because the phenol will do the job of nuclease inactivation.

- To capture more amount of mRNA, modify the amount of lysis buffer :ethanol (from 1:1 to 1:1.5) during the binding step.
Downstream tips
- Do vortex for 2-plus minutes during Acid-Phenol:Chloroform extraction step. This will facilitate the removal of tightly bound proteins typically associated with RNA.

Publication protocol

Total RNA was purified using the mirVana™ miRNA Isolation Kit (ABI, Carlsbad, CA USA). For miRNA profiling, we utilized the 5th generation (human, mouse, rat) miRCURY LNA™ microRNA Array Kit (Exiqon Inc., Woburn, MA USA). Total RNA recovered from three independent experiments were quality checked using 1% formaldehyde-agarose gel electrophoresis and an Agilent bioanalyzer, and the microarrays were processed (i.e. samples labeled, arrays hybridized, washed and scanned) by the UCLA Clinical Microarray Core facility (http://www.pathology.ucla.edu/cmc).

Full paper   Login or join for free to view the full paper.

Reviews

mirVana™ miRNA Isolation Kit, with phenol from Thermo Fisher Scientific has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary human osteoblasts using mirVana™ miRNA Isolation Kit, with phenol from Thermo Fisher Scientific.

Paper title
Vitamin D activation of functionally distinct regulatory miRNAs in primary human osteoblasts
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for mirVana™ miRNA Isolation Kit, with phenol below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Cells - primary human osteoblasts using mirVana™ miRNA Isolation Kit, with phenol from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms